Scientific Literature

 

Previstage™ GCC Colorectal Cancer Staging Test

Mejia A, and Waldman S. Previstage™ GCC test for staging patients with colorectal cancer. Expert Reviews 2008; 8 (5): 571-578.

Biology of Guanylyl Cyclase C (GCC or GUCY2C)

  1. Lucas KA, Pitari GM, Kazerounian S, Ruiz-Stewart I, Park J, Schulz S, Chepenik KP, Waldman SA. Guanylyl cyclases and signaling by cyclic GMP. Pharmacol Rev. 2000 Sep;52(3):375-414.
  2. Pearlman JM, Prawer SP, Barber MT, Parkinson SJ, Schulz S, Park J, Zook M, Waldman SA. A splice variant of the transcript for guanylyl cyclase C is expressed in human colon and colorectal cancer cells. Dig Dis Sci. 2000 Feb;45(2):298-305.
  3. Schulz S, Waldman SA. The guanylyl cyclase family of natriuretic peptide receptors. Vitam Horm. 1999;57:123-51.
  4. Waldman SA, Barber M, Pearlman J, Park J, George R, Parkinson SJ. Heterogeneity of guanylyl cyclase C expressed by human colorectal cancer cell lines in vitro. Cancer Epidemiol Biomarkers Prev. 1998 Jun;7(6):505-14.
  5. Deshmane SP, Parkinson SJ, Crupper SS, Robertson DC, Schulz S, Waldman SA. Cytoplasmic domains mediate the ligand-induced affinity shift of guanylyl cyclase C. Biochemistry. 1997 Oct 21;36(42):12921-9.
  6. Parkinson SJ, Alekseev AE, Gomez LA, Wagner F, Terzic A, Waldman SA. Interruption of Escherichia coli heat-stable enterotoxin-induced guanylyl cyclase signaling and associated chloride current in human intestinal cells by 2-chloroadenosine. J Biol Chem. 1997 Jan 10;272(2):754-8.
  7. Parkinson SJ, Waldman SA. An intracellular adenine nucleotide binding site inhibits guanyly cyclase C by a guanine nucleotide-dependent mechanism. Biochemistry. 1996 Mar 12;35(10):3213-21.
  8. Urbanski R, Carrithers SL, Waldman SA. Internalization of E. coli ST mediated by guanylyl cyclase C in T84 human colon carcinoma cells. Biochim Biophys Acta. 1995 Aug 17;1245(1):29-36.
  9. Deshmane SP, Carrithers SL, Parkinson SJ, Crupper SS, Robertson DC, Waldman SA. Rat guanylyl cyclase C expressed in COS-7 cells exhibits multiple affinities for Escherichia coli heat-stable enterotoxin. Biochemistry. 1995 Jul 18;34(28):9095-102.
  10. Carrithers SL, Parkinson SJ, Goldstein S, Park P, Robertson DC, Waldman SA. Escherichia coli heat-stable toxin receptors in human colonic tumors. Gastroenterology. 1994 Dec;107(6):1653-61.
  11. Parkinson SJ, Carrithers SL, Waldman SA. Opposing adenine nucleotide-dependent pathways regulate guanylyl cyclase C in rat intestine. J Biol Chem. 1994 Sep 9;269(36):22683-90.
  12. Leitman DC, Waldman SA, Murad F. Regulation of particulate guanylate cyclase by natriuretic peptides and Escherichia coli heat-stable enterotoxin. Adv Pharmacol. 1994;26:67-86.
  13. Hakki S, Robertson DC, Waldman SA. A 56 kDa binding protein for Escherichia coli heat-stable enterotoxin isolated from the cytoskeleton of rat intestinal membrane does not possess guanylate cyclase activity. Biochim Biophys Acta. 1993 Oct 10;1152(1):1-8.
  14. Hakki S, Crane M, Hugues M, O'Hanley P, Waldman SA. Solubilization and characterization of functionally coupled Escherichia coli heat-stable toxin receptors and particulate guanylate cyclase associated with the cytoskeleton compartment of intestinal membranes. Int J Biochem. 1993 Apr;25(4):557-66.
  15. Crane MR, Hugues M, O'Hanley PD, Waldman SA. Identification of two affinity states of low affinity receptors for Escherichia coli heat-stable enterotoxin: correlation of occupation of lower affinity state with guanylate cyclase activation. Mol Pharmacol. 1992 Jun;41(6):1073-80.
  16. Hugues M, Crane MR, Thomas BR, Robertson D, Gazzano H, O'Hanley P, Waldman SA. Affinity purification of functional receptors for Escherichia coli heat-stable enterotoxin from rat intestine. Biochemistry. 1992 Jan 14;31(1):12-6.
  17. Hugues M, Crane M, Hakki S, O'Hanley P, Waldman SA. Identification and characterization of a new family of high-affinity receptors for Escherichia coli heat-stable enterotoxin in rat intestinal membranes. Biochemistry. 1991 Nov 5;30(44):10738-45.
  18. Gazzano H, Wu HI, Waldman SA. Activation of particulate guanylate cyclase by Escherichia coli heat-stable enterotoxin is regulated by adenine nucleotides. Infect Immun. 1991 Apr;59(4):1552-7.
  19. Crane MR, O'Hanley P, Waldman SA. Rat intestinal cell atrial natriuretic peptide receptor coupled to guanylate cyclase. Gastroenterology. 1990 Jul;99(1):125-31.
  20. Ivens K, Gazzano H, O'Hanley P, Waldman SA. Heterogeneity of intestinal receptors for Escherichia coli heat-stable enterotoxin. Infect Immun. 1990 Jun;58(6):1817-20.
  21. Waldman SA, O'Hanley P. Influence of a glycine or proline substitution on the functional properties of a 14-amino-acid analog of Escherichia coli heat-stable enterotoxin. Infect Immun. 1989 Aug;57(8):2420-4.
  22. Carr S, Gazzano H, Waldman SA. Regulation of particulate guanylate cyclase by Escherichia coli heat-stable enterotoxin: receptor binding and enzyme kinetics. Int J Biochem. 1989;21(11):1211-5.
  23. Kuno T, Kamisaki Y, Waldman SA, Gariepy J, Schoolnik G, Murad F. Characterization of the receptor for heat-stable enterotoxin from Escherichia coli in rat intestine. J Biol Chem. 1986 Jan 25;261(3):1470-6.
  24. Waldman SA, Kuno T, Kamisaki Y, Chang LY, Gariepy J, O'Hanley P, Schoolnik G, Murad F. Intestinal receptor for heat-stable enterotoxin of Escherichia coli is tightly coupled to a novel form of particulate guanylate cyclase. Infect Immun. 1986 Jan;51(1):320-6.

Use of Guanylyl Cyclase C (GCC or GUCY2C) as a Diagnostic Marker

  1. Waldman, SA, Hyslop, T, Schulz, S, Barkun, A, Nielsen, K, Haaf, J, Bonaccorso, C, Li Y, Weinberg, DS. Association of GUCY2C Expression in Lymph Nodes With Time to Recurrence and Disease-Free Survival in pN0 Colorectal Cancer. JAMA. 2009;301(7):745-752.
  2. Snook AE, Eisenlohr LC, Rothstein JL, Waldman SA. Cancer mucosa antigens as a novel immunotherapeutic class of tumor-associated antigen. Clin Pharmacol Ther. 2007 Dec;82(6):734-9.
  3. Waldman SA, Terzic A. Translating MicroRNA discovery into clinical biomarkers in cancer. JAMA. 2007 May 2;297(17):1923-5.
  4. Fortina P, Kricka LJ, Graves DJ, Park J, Hyslop T, Tam F, Halas N, Surrey S, Waldman SA. Applications of nanoparticles to diagnostics and therapeutics in colorectal cancer. Trends Biotechnol. 2007 Apr;25(4):145-52.
  5. Wilson C, Schulz S, Waldman SA.  Biomarker development, commercialization, and regulation: individualization of medicine lost in translation. Clin Pharmacol Ther. 2007 Feb;81(2):153-5.
  6. Waldman SA, Christensen NB, Moore JE, Terzic A. Clinical pharmacology: the science of therapeutics. Clin Pharmacol Ther. 2007 Jan;81(1):3-6.
  7. Waldman SA, Fortina P, Surrey S, Hyslop T, Kricka LJ, Graves DJ. Opportunities for near-infrared thermal ablation of colorectal metastases by guanylyl cyclase C-targeted gold nanoshells. Future Oncol. 2006 Dec;2(6):705-16.
  8. Schulz S, Hyslop T, Haaf J, Bonaccorso C, Nielsen K, Witek ME, Birbe R, Palazzo J, Weinberg D, Waldman SA. A validated quantitative assay to detect occult micrometastases by reverse transcriptase-polymerase chain reaction of guanylyl cyclase C in patients with colorectal cancer. Clin Cancer Res. 2006 Aug 1;12(15):4545-52.
  9. Frick GS, Pitari GM, Weinberg DS, Hyslop T, Schulz S, Waldman SA. Guanylyl cyclase C: a molecular marker for staging and postoperative surveillance of patients with colorectal cancer. Expert Rev Mol Diagn. 2005 Sep;5(5):701-13.
  10. Birbe R, Palazzo JP, Walters R, Weinberg D, Schulz S, Waldman SA. Guanylyl cyclase C is a marker of intestinal metaplasia, dysplasia, and adenocarcinoma of the gastrointestinal tract. Hum Pathol. 2005 Feb;36(2):170-9.
  11. Gelmann A, Desnoyers R, Cagir B, Weinberg D, Boman BM, Waldman SA. Colorectal cancer staging and adjuvant chemotherapy. Expert Opinion on Pharmacother. 2000 May;1(4):737-55.
  12. Cagir B, Gelmann A, Park J, Fava T, Tankelevitch A, Bittner EW, Weaver EJ, Palazzo JP, Weinberg D, Fry RD, Waldman SA. Guanylyl cyclase C messenger RNA is a biomarker for recurrent stage II colorectal cancer. Ann Intern Med. 1999 Dec 7;131(11):805-12.
  13. Waldman SA, Cagir B, Rakinic J, Fry RD, Goldstein SD, Isenberg G, Barber M, Biswas S, Minimo C, Palazzo J, Park PK, Weinberg D. Use of guanylyl cyclase C for detecting micrometastases in lymph nodes of patients with colon cancer. Dis Colon Rectum. 1998 Mar;41(3):310-5.
  14. Carrithers SL, Barber MT, Biswas S, Parkinson SJ, Park PK, Goldstein SD, Waldman SA. Guanylyl cyclase C is a selective marker for metastatic colorectal tumors in human extraintestinal tissues. Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14827-32.
  15. Carrithers SL, Parkinson SJ, Goldstein SD, Park PK, Urbanski RW, Waldman SA. Escherichia coli heat-stable enterotoxin receptors. A novel marker for colorectal tumors. Dis Colon Rectum. 1996 Feb;39(2):171-81.
  16. Waldman SA, O'Hanley P, Falkow S, Schoolnik G, Murad F. A simple, sensitive, and specific assay for the heat-stable enterotoxin of Escherichia coli. J Infect Dis. 1984 Jan;149(1):83-9.

Use of Guanylyl Cyclase C (GCC or GUCY2C) as a Possible Therapeutic Target

  1. Li P, Lin JE, Chervoneva I, Schulz S, Waldman SA, Pitari GM. Homeostatic control of the crypt-villus axis by the bacterial enterotoxin receptor guanylyl cyclase C restricts the proliferating compartment in intestine. Am J Pathol. 2007 Dec;171(6):1847-58.
  2. Pitari GM, Li P, Lin JE, Zuzga D, Gibbons AV, Snook AE, Schulz S, Waldman SA. The paracrine hormone hypothesis of colorectal cancer. Clin Pharmacol Ther. 2007 Oct;82(4):441-7.
  3. Li P, Schulz S, Bombonati A, Palazzo JP, Hyslop TM, Xu Y, Baran AA, Siracusa LD, Pitari GM, Waldman SA. Guanylyl cyclase C suppresses intestinal tumorigenesis by restricting proliferation and maintaining genomic integrity. Gastroenterology. 2007 Aug;133(2):599-607.
  4. Pitari GM, Li T, Baksh RI, Waldman SA. Exisulind and guanylyl cyclase C induce distinct antineoplastic signaling mechanisms in human colon cancer cells. Mol Cancer Ther. 2006 May;5(5):1190-6.
  5. Debruyne PR, Witek M, Gong L, Birbe R, Chervoneva I, Jin T, Domon-Cell C, Palazzo JP, Freund JN, Li P, Pitari GM, Schulz S, Waldman SA. Bile acids induce ectopic expression of intestinal guanylyl cyclase C Through nuclear factor-kappaB and Cdx2 in human esophageal cells. Gastroenterology 2006 Apr;130(4):1191-206.
  6. Pitari GM, Zingman LV, Hodgson DM, Alekseev AE, Kazerounian S, Bienengraeber M, Hajnóczky G, Terzic A, Waldman SA. Bacterial enterotoxins are associated with resistance to colon cancer. Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2695-9.
  7. Park J, Schulz S, Haaf J, Kairys JC, Waldman SA. Ectopic expression of guanylyl cyclase C in adenocarcinomas of the esophagus and stomach. Cancer Epidemiol Biomarkers Prev. 2002 Aug;11(8):739-44.
  8. Ruiz-Stewart I, Kazerounian S, Pitari GM, Schulz S, Waldman SA. Soluble guanylate cyclase is allosterically inhibited by direct interaction with 2-substituted adenine nucleotides. Eur J Biochem. 2002 Apr;269(8):2186-93.
  9. Wolfe HR, Waldman SA. A comparative molecular field analysis (COMFA) of the structural determinants of heat-stable enterotoxins mediating activation of guanylyl cyclase C. J Med Chem. 2002 Apr 11;45(8):1731-4.
  10. Wolfe HR, Mendizabal M, Lleong E, Cuthbertson A, Desai V, Pullan S, Fujii DK, Morrison M, Pither R, Waldman SA. In vivo imaging of human colon cancer xenografts in immunodeficient mice using a guanylyl cyclase C--specific ligand. J Nucl Med. 2002 Mar;43(3):392-9.
  11. Kazerounian S, Pitari GM, Ruiz-Stewart I, Schulz S, Waldman SA. Nitric oxide activation of soluble guanylyl cyclase reveals high and low affinity sites that mediate allosteric inhibition by calcium. Biochemistry. 2002 Mar 12;41(10):3396-404.
  12. Di Guglielmo MD, Park J, Schulz S, Waldman SA. Nucleotide requirements for CDX2 binding to the cis promoter element mediating intestine-specific expression of guanylyl cyclase C. FEBS Lett. 2001 Oct 26;507(2):128-32.
  13. Fava TA, Desnoyers R, Schulz S, Park J, Weinberg D, Mitchell E, Waldman SA. Ectopic expression of guanylyl cyclase C in CD34+ progenitor cells in peripheral blood. J Clin Oncol. 2001 Oct 1;19(19):3951-9.
  14. Pitari GM, Di Guglielmo MD, Park J, Schulz S, Waldman SA. Guanylyl cyclase C agonists regulate progression through the cell cycle of human colon carcinoma cells. Proc Natl Acad Sci U S A. 2001 Jul 3;98(14):7846-51.
  15. Park J, Schulz S, Waldman SA. Intestine-specific activity of the human guanylyl cyclase C promoter is regulated by Cdx2. Gastroenterology. 2000 Jul;119(1):89-96.
  16. Parkinson SJ, Jovanovic A, Jovanovic S, Wagner F, Terzic A, Waldman SA. Regulation of nitric oxide-responsive recombinant soluble guanylyl cyclase by calcium. B